T4026
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 | Immunofluorescence Human monocytes cultured on hardware MIA in real microgravity on board International Space Station, labeled with Monoclonal Anti-β-Tubulin, clone TUB 2.1 (Cat. No. T4026) (green) and DAP for the DNA. From Giuseppe Pani, Dipartmento di Scienze Fisiologiche, Biochimiche e Cellulari, Sassari University, Sassari, Italy. |
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T4026
Sigma
Monoclonal Anti-β-Tubulin antibody produced in mouse
clone TUB 2.1, ascites fluid
| Synonym: | Monoclonal Anti-β-Tubulin |
Description
| Immunogen | tubulin from rat brain. |
| Specificity | The antibody recognizes all five isoforms of β-tubulin (β1-β5).1 It reacts with the β-Lc and β-Sc fragments in the carboxy-terminal part of β−tubulin in immunoblotting. The antibody may be used to localize β−tubulin in cultured cells or tissue sections. |
Properties
| antibody form | ascites fluid |
| clone | TUB 2.1 (monoclonal) |
| mol wt | antigen mol wt 55 kDa |
| contains | 15 mM sodium azide |
| species reactivity | chicken, human, frog, wheat, plant, rabbit, bovine, mouse, hamster, moth, rat, sea urchin |
| application(s) | immunoblotting: suitable |
| indirect immunofluorescence: 1:200 using cultured chicken fibroblasts | |
| isotype | IgG1 |
| Gene Information | human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) |
| rat ... Tubb2(29212), Tubb2c(296554) | |
| mouse ... Tubb1(104068), Tubb2a(22151), Tubb2c(227613) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| Cited Reference | 1. Wolff, A., et al. Biol. Cell 63, 319-326, (1988) Abstract |
| reference | Andreo, C.S., et al., Leaf Development in the Single-Cell C4 System in Bienertia sinuspersici: Expression of Genes and Peptide Levels for C4 Metabolism in Relation to Chlorenchyma Structure under Different Light Conditions Plant Physiol. 148, 593-610, (2008) Abstract |
| Murchison, E.P., et al., Critical roles for Dicer in the female germline Genes Dev. 21, 682-693, (2007) Abstract | |
| De Rooij, D.G., et al., ATM Promotes the Obligate XY Crossover and both Crossover Control and Chromosome Axis Integrity on Autosomes PLoS Genet. 4, e1000076, (2008) Abstract | |
| Qian Li-Wu, et al., Kaposi's Sarcoma-Associated Herpesvirus Disrupts Adherens Junctions and Increases Endothelial Permeability by Inducing Degradation of VE-Cadherin J. Virol. 82, 11902-11912, (2008) | |
| Gozes, I., et al., Monoclonal antibodies that recognize discrete forms of tubulin. Proc. Natl. Acad. Sci. U. S. A. 79, 2579, (1982) | |
| Matthes, T., et al., Antitubulin antibodies. II. Natural autoantibodies and induced antibodies recognize different epitopes on the tubulin molecule. J. Immunol. 141, 3135, (1988) | |
| Schomai, A., and Shaul, Y. Hepatology 37, 764-770, (2003) Abstract | |
| Soroka, C.J., et al. J. Biol. Chem. 274, 26416-26424, (1999) | |
| Gingras, D., et al. J. Biol. Chem. 271, 12807-12813, (1996) | |
| Binarova, P., et al. Plant Cell 12, 433-442, (2000) Abstract |
